Dr. Craig Gatto
Interim chair of chemistry
Office
JH Julian Hall 214
Office Phone
Email
- About
- Education
- Awards & Honors
- Selected Research
Research Interests & Areas
The active transport of ions across cell membranes is performed by P-type ATPases. These are integral membrane proteins which use the energy of hydrolysis of ATP to pump ions across biological membranes. The mechanism by which these proteins couple ATP hydrolysis to ion transport is one of the central interests of the laboratory. My laboratory focuses on structure-function studies, molecular mechanism, assembly and subcellular trafficking of these important transporters. P-type pumps are tansiently phosphorylated from ATP and transport alkali metals (e.g. Na+, K+, H+, Ca2+) as well as heavy metals (e.g Cu2+ and Cd2+). Members of this protein superfamily are represented across all phyla.
Ph D Physiology
University of Missouri-Columbia
Columbia MO
MS Zoology
Eastern Illinois University
Charleston, IL
BS Zoology
Eastern Illinois University
Charleston, IL
University Professor
Illinois State University
2015
Outstanding University Researcher Award
Illinois State University
2009
Faculty Star
ISU Men's Basketball game
2009
2008 Class of Outstanding Graduate Alumni
Eastern Illinois University Graduate School
2008
Outstanding College Researcher Award
Illinois State University
2008
Outstanding College Service Award
College of Arts and Sciences
2007
University Research Initiative Award
Illinois State University
2001
James Metcalfe Outstanding Reserach Proposal
American Heart Association
1996
James Metcalfe Outstanding Reserach Proposal
American Heart Association
1995
Student Reseracher Award
University of Missouri-Columbia
1993
Outstanding Gradaute Researcher Award
Eastern Illinois University
1989
Grants and Contracts
Cell Physiology of the Na,K-ATPase
Craig Gatto.
National Institutes of Health. June 1 2016 - May 31 2019
Branched-chain fatty acids and membrane function in Listeria monocytogenes
Craig Gatto, Brian James Wilkinson.
National Institutes of Health. September 1 2013 - August 31 2016
Cellular structure-function of Na pump Assembly-
Craig Gatto.
National Institutes of Health- R15. March 1 2009 - February 28 2012
Role for Na,K-ATPase in Nucleoplasmic Ca Homeostasis
Craig Gatto.
National Institutes of Health- R21. July 1 2009 - June 30 2012
Experiment
Craig Gatto.
May 01 2008 - May 20 2010
Bridges Coordinator
Craig Gatto, Louis DeFelice.
National Institutes of Health-R25. July 1 2007 - June 30 2008
Acquisition of a Microarray Scanner for Illinois State University
Craig Gatto.
National Science Foundation-MRI. January 1 2005 - December 31 2007
Funtional Analysis of the Tomato Ca-ATPase Mechanism
Craig Gatto.
National Science Foundation- CAREER. February 1 2005 - January 30 2010
Mechanism of Artemesinin Antiparasitic Activity
Craig Gatto, David Williams.
National Institutes of Health- R21. November 1 2005 - October 31 2008
Instructor's Manual
The reactive nitrogen species peroxynitrite is a potent inhibitor of renal Na,K-ATPase activity
M S Reifenberger, K L Arnett, Craig Gatto, M A Milanick.
Am. J. Physiol. -Renal Physiol, 295, 1191-1198, (2008)
Journal Article
FXYD protein isoforms differentially modulate human Na/K pump function.
Dylan Meyer, Sharan Bijlani, Marilina DeSautu, Victoria Young, Kerri Spontarelli, Craig Gatto, Pablo Artigas.
152 (12), 660-672, (2020), 10.1085/jgp.202012660
Lipidomic and Ultrastructural Characterization of Cell Envelope of Staphylococcus aureus Grown in the Presence of Human Serum
Kell M Hines, Gloria Alvarado, X Chen, Craig Gatto, Antje Pokorny, F Alonzo, Brian James Wilkinson, Libin Xu.
mSphere, 5 (3), 339-355, (2020)
Plasticity of Coagulase-Negative Staphylococcal Membrane Fatty Acid Composition and Implications for Responses to Antimicrobial Agents.
Kiran B Tiwari, Craig Gatto, Brian James Wilkinson.
Antibiotics, 9 (214), 1-10, (2020)
External Ion Access in the Na/K Pump: Kinetics of Na<sup>+</sup>, K<sup>+</sup>, and Quaternary Amine Interaction.
K S Stanley, V C Young, Craig Gatto, P Artigas.
Biophysical journal, 115 (2), 361-374, (2018), 10.1016/j.bpj.2018.06.007
Interrelationships between Fatty Acid Composition, Staphyloxanthin Content, Fluidity, and Carbon Flow in the <i>Staphylococcus aureus</i> Membrane.
K B Tiwari, Craig Gatto, Brian James Wilkinson.
Molecules (Basel, Switzerland), 23 (5), (2018), 10.3390/molecules23051201
Roles of pyruvate dehydrogenase and branched-chain α-keto acid dehydrogenase in branched-chain membrane fatty acid levels and associated functions in Staphylococcus aureus.
V K Singh, S Sirobhushanam, R P Ring, S Singh, Craig Gatto, Brian James Wilkinson.
Journal of medical microbiology, 67 (4), 570-578, (2018), 10.1099/jmm.0.000707
On the effect of hyperaldosteronism-inducing mutations in Na/K pumps.
D J Meyer, Craig Gatto, P Artigas.
The Journal of general physiology, 149 (11), 1009-1028, (2017), 10.1085/jgp.201711827
Utilization of multiple substrates by butyrate kinase from Listeria monocytogenes.
S Sirobhushanam, Charitha Srinivas Reddy Galva, L P Saunders, S Sen, Radheshyam Jayaswal, Brian James Wilkinson, Craig Gatto.
Biochimica et biophysica acta, 1862 (3), 283-290, (2017), 10.1016/j.bbalip.2016.12.001
Broad substrate specificity of phosphotransbutyrylase from Listeria monocytogenes: A potential participant in an alternative pathway for provision of acyl CoA precursors for fatty acid biosynthesis.
S Sirobhushanam, Charitha Srinivas Reddy Galva, S Sen, Brian James Wilkinson, Craig Gatto.
Biochimica et biophysica acta, 1861 (9 Pt A), 1102-1110, (2016), 10.1016/j.bbalip.2016.06.003
Growth-Environment Dependent Modulation of Staphylococcus aureus Branched-Chain to Straight-Chain Fatty Acid Ratio and Incorporation of Unsaturated Fatty Acids.
S Sen, S Sirobhushanam, S R Johnson, Y Song, R Tefft, Craig Gatto, Brian James Wilkinson.
PloS one, 11 (10), e0165300, (2016), 10.1371/journal.pone.0165300
Insights into the Mechanism of Homeoviscous Adaptation to Low Temperature in Branched-Chain Fatty Acid-Containing Bacteria through Modeling FabH Kinetics from the Foodborne Pathogen Listeria monocytogenes.
L P Saunders, S Sen, Brian James Wilkinson, Craig Gatto.
Frontiers in microbiology, 7, 1386, (2016), 10.3389/fmicb.2016.01386
Intracellular Requirements for Passive Proton Transport through the Na<sup>+</sup>,K<sup>+</sup>-ATPase.
K S Stanley, D J Meyer, Craig Gatto, P Artigas.
Biophysical journal, 111 (11), 2430-2439, (2016), 10.1016/j.bpj.2016.09.042
Bone-Remodeling Transcript Levels Are Independent of Perching in End-Of- Lay White Leghorn Chickens.
Maurice Dale, Erin Mortimer, Santharam Kolli, Erik Achramowicz, Glen Borchert, Steven Anthony Juliano, Scott Halkyard, Nick Seitz, Craig Gatto, Patricia Hester, David Alan Rubin.
International Journal of Molecular Sciences, 16, 2663-2677, (2015)
Inhibition of the Na,K-ATPase by the acetaminophen metabolite N-acetylbenzoquinoneimine.
Craig Gatto, Jeff B. Helms, Jamie Meyer, Charles J. Costa, Eric Plowman, Jeremy P. Holden.
J. Integrative & Mol. Physiol., 5, 1-17, (2015)
Short branched-chain C6 carboxylic acids result in increased growth, novel 'unnatural' fatty acids and increased membrane fluidity in a Listeria monocytogenes branched-chain fatty acid-deficient mutant.
S Sen, S Sirobhushanam, M P Hantak, P Lawrence, J T Brenna, Craig Gatto, Brian James Wilkinson.
Biochimica et biophysica acta, 1851 (10), 1406-15, (2015), 10.1016/j.bbalip.2015.07.006
Sodium and Proton Effects on Inward Proton Transport Through Na/K pumps.
Craig Gatto, Travis J. Mitchell, Camila Zugarramurdi, J. Fernando Olivera, Pablo Artigas.
Biophysical Journal, 106, 2555 - 2565, (2014)
ATP protects against FITC labeling of Solanum lycopersicon and Arabidopsis thaliana Ca2+-ATPase ATP binding domains.
C Galva, G K Virgin, J B Helms, Craig Gatto.
Plant physiology and biochemistry : PPB / Societe francaise de physiologie vegetale, 71, 261-7, (2013), 10.1016/j.plaphy.2013.07.010
Characterization of potential drug targets farnesyl diphosphate synthase and geranylgeranyl diphosphate synthase in Schistosoma mansoni.
P D Ziniel, J Desai, C L Cass, Craig Gatto, E Oldfield, D L Williams.
Antimicrobial agents and chemotherapy, 57 (12), 5969-76, (2013), 10.1128/AAC.00699-13
Nuclear Na+/K+-ATPase plays an active role in nucleoplasmic Ca2+ homeostasis.
C Galva, P Artigas, Craig Gatto.
Journal of cell science, 125 (Pt 24), 6137-47, (2012), 10.1242/jcs.114959
Soymilk: an effective and inexpensive blocking agent for immunoblotting.
C Galva, Craig Gatto, M Milanick.
Analytical biochemistry, 426 (1), 22-3, (2012), 10.1016/j.ab.2012.03.028
Comprehensive Analysis of MicroRNA Genomic Loci Identifies Pervasive Repetitive-Element Orgins
G M Borchert, N W Holton, J D Williams, I. Bishop, J A Dembosky, J E Elste, N S Gregoire, W L Hernan, J A Kim, W W Koehler, J C Lengerich, A A Medema, M A Nguyen, G D Ower, M A Rarick, B N Strong, N J Tardi, N M Tasker, D J Wozniak, Craig Gatto, Erik D Larson.
Mob. Gen. Elements, 1 (1), (2011)
NO control: nitric oxide directly regulates substrate delivery to NOS.
Craig Gatto.
Am. J. Physiol. - Cell Physiology, 299, C213 - C215, (2010)
Selectivity of externally facing ion-binding sites in the Na/K pump to alkali metals and organic cations.
Craig Gatto, Gail K. Virgin, Ian M. Ratheal, Pablo Artigas.
Proc. Natl. Acad. Sci., 107, 18718 - 18723, (2010)
A Tomato ER-type Ca2+-ATPase, LCA1,
N A Johnson, F Liu, P D Weeks, A E Hentzen, H P Kruse, J J Parker, M Laursen, P Nissen, C J Costa, Craig Gatto.
Arch. Biochem. Biophysics, 481, 157-168, (2009)
Presentations
Deciphering the molecular mechanism of active transport via P-type ATPases
Craig Gatto.
School of Biological Sciences Seminar Series, Normal, IL, August 4, 2009
β-Ketoacyl-Acyl Carrier Protein Synthase III (FabH) of Listeria monocytogenes has Increased Preference for the Precursor of Anteiso Fatty Acids over Iso Branched-Chain Fatty Acid Precursors at Low Temperature
Mrittika Sengupta, Vaibhav Jain, Atul Singh, Craig Gatto, Radheshyam Jayaswal, C O Rock, Brian James Wilkinson.
109th General Meeting of the American Society for Microbiology, Philadelphia, PA, May, 2009
Altered transport in Na/K pumps with -subunits truncated at its 5 C-terminal residues
Siddhartha Yaragatupalli, Craig Gatto, Pablo Artigas.
Experimental Biology, New Orlean, LA, April 17, 2009
Expression Pattern of Fluorescently Tagged Na pump in C. elegans
C L Templeton, A J Otsuka, M Lee, J Ahn, Craig Gatto.
Biophysical Society Meeting, Long Beach, CA, , 2008
In vivo monitoring of Na,K-ATPase trafficking in polarized epithelium
C L Templeton, A J Otsuka, M Lee, J Ahn, Craig Gatto.
Annual Biomedical Research Conference for Minority Students, Orlando, FL, November, 2008
Extracellular protons alter cation binding to the red cell Na,K-ATPase
Mark A Milanick, Craig Gatto.
Red Cell Conference, Rochester, NY, October 25, 2008
Cellular Differences between the Tomato Plant SERCA Ortholog, LCA, Compared to its Vertebrate Counterparts
N A Johnson, M E Doehring, P D Weeks, F Liu, H P Kruse, Craig Gatto.
12th International Meeting on P-type ATPases, Aarhus, Denmark, August, 2008
Functional differences between plant and animal ER-type Ca-ATPases
Craig Gatto.
Centre for Membrane Pumps in Cells and Disease, Dept. of Molecular Biology Seminar, Aarhus, Denmark, August 16, 2008
Pharmacological dissection of the Na pump molecular mechanism
Craig Gatto.
Dept. of Molecular Physiology & Biophysics Seminar, Lubbock, TX, May 10, 2008
Deciphering the molecular mechanism of active transport via P-type ATPases
Craig Gatto.
Department of Physics Seminar, Normal, IL, April 22, 2008
Differential localization in root cells of two homologous plant Ca+2-ATPases, LCA and ECA1
Mark Doehring, Craig Gatto.
Biophysical Society Meeting, Baltimore, MD, March 5, 2007
Inhibition of the Na,K-ATPase by the acetaminophen metabolite N-acetylbenzoquinoneimine
Jeff B Helms, Jamie Meyer, Charles J Costa, Jeremy P Holden, Craig Gatto.
Biophysical Society Meeting, Baltimore, MD, March 5, 2007
The Extracellular Domain of the Na pump Beta-subunit is sufficient for Alpha-Beta Assembly and Delivery to the PM
Gail K Virgin, Jeff B Helms, Charles J Costa, Craig Gatto.
Biophysical Society Meeting, Baltimore, MD, March 5, 2007